WebJan 7, 2024 · The fragment containing weak phosphoglycerate kinase (PGK) promoter with cutting sites was obtained from pCDH-PGK (Plasmid #72268, Addgene, Watertown, MA, USA) by digestion with EcoRI/HpaI restriction enzyme and replaced the CMV promoter in pCDH-CMV-PDGF-mRFP lentiviral expression vector by with EcoRI/HpaI digestion and … WebSep 5, 2024 · Mouse phosphoglycerate kinase 1 (PGK) promoter was also used to drive transgene expression in different cell lines 23, 24. Besides natural promoters, hybrid …
Efficient expression of the Saccharomyces cerevisiae PGK gene
Web1 Introduction. The promoter of the yeast gene encoding the glycolytic enzyme phosphoglycerate kinase (PGK) has been used to construct vectors for expression of … Webphosphoglycerate kinase (PGK) promoter throughout development to hematopoietic cells in vitro. An oncoret-rovirus vector containing the MESV LTR and the GFP gene was used for comparison. Fluorescence-activated cell sorting analysis of transduced CCE ES cells showed 99.8 and 86.7% GPF-expressing ES cells in the VSV-G- fishing rod holders for backpacks
Constitutive Expression Vectors: PGK SpringerLink
WebSep 3, 2024 · Phosphoglycerate kinase 1 (PGK1) is the first enzyme in glycolysis to generate a molecule of ATP in the conversion of 1,3-bisphosphoglycerate (1,3-BPG) to 3-phosphoglycerate (3-PG). In addition to the role of glycolysis, PGK-1 acts as a polymerase alpha cofactor protein, with effects on the tricarboxylic acid cycle, DNA replication and … WebMar 31, 2024 · Introduction. Phosphoglycerate kinase 1 (PGK-1) is a key enzyme in the glycolytic pathway and is encoded by PGK-1 (Online Mendelian Inheritance in Man (OMIM) #311800), which maps to the X chromosome and is expressed in all somatic tissues. PGK-1 deficiency (OMIM #300653) is an uncommon cause of congenital nonspherocytic … WebPurpose 5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expression Depositing Lab Kryn Stankunas Publication Fowler et al PLoS One. 2016 Aug 8;11 (8):e0159277. doi: 10.1371/journal.pone.0159277. eCollection 2016. ( How to cite ) Sequence Information Sequences (3) Ordering canceling a webex meeting