Dylight 550 spectra

WebCatalog number: 62262. Thermo Scientific DyLight 550 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight 550 used to fluorescently label … DyLight 550 provides vibrant orange-to-red fluorescence with better performance … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebThermo Scientific DyLight 550 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 550 used to fluorescently label sulfhydryl-containing …

A Guide to Fluorochromes - Abcam

WebAbout DyLight 550. DyLight™ 550 is a yellow-emitting fluorophore that can be excited by the 561 nm yellow-green laser and collected using a 582/15 nm bandpass filter. DyLight … Web10 1040.06 0.139 mg of DyLight 550 NHS . Ester 150,000 MW 2 mg IgG × × = 13.9. μl of DyLight 550 NHS Ester 1 mg 100 μl 0.139 mg of DyLight 550 NHS Ester × = D. … greens and ham hock recipe https://luniska.com

Mouse Anti-DYKDDDDK IgG: DyLight® 550;100ug

WebMar 6, 2024 · IHC-P. 1/50 - 1/500. DyLight ® 550 replaces DyLight ® 549. The DyLight ® 550 excitation is 562nm and the emission is 576nm. DyLight ® 550 provides the same orange-to-red fluorescence and photostability as the DyLight ® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight ® 549 or DyLight ® 550. WebThe DyLight® fluorophore spectra are shown below. Table: Spectral properties of DyLight® fluorochromes. In order to expand the spectra, please click on the spectrum … WebDyLight 550 emits vibrant orange-to-red fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 556nm, high fluorescence at 584nm (extinction coefficient 1.5 x105 cm-1M-1) and high quantum yield. DyLight dyes are extremely bright and are highly water soluble meaning that a high dye-protein ratio ... greens and gyros somers point

Frequently asked questions - DyLight® 550 repla

Category:Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight™ 550

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Dylight 550 spectra

Spectrum [DyLight 405] AAT Bioquest

WebBrowse Toyota Prius vehicles in Chillum, MD for sale on Cars.com, with prices under $10,000. Research, browse, save, and share from 22 Prius models in Chillum, MD. WebProtein A DyLight™680 has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight ...

Dylight 550 spectra

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WebJan 21, 2010 · Absorption and fluorescence emission spectra of DyLight 594 free and labeled on IgG are presented in Fig. 1.This dye can be conveniently excited from 530 to 610 nm.Many excitation sources like a green 532 nm, 543 nm and 568 nm lasers, as well as Rhodamine 6G dye laser operate in this spectral region.Most types of photodetectors are … WebForm / Storage: Conjugate supplied as a liquid in 0.1M sodium phosphate, 0.15M NaCl (pH7.4), 1% BSA, 0.02% NaN3. Upon receipt, store at 2-8°C in the dark.

WebJan 10, 2024 · TRITC is excited with light in the green spectrum with a maximum at 550 nm. Its emission maximum is lying at 573 nm. The bond to proteins (e.g. antibodies) is also based on a reactive isothiocyanate group. ... DyLight 405: 399 nm: 434 nm: DyLight 350: 332 nm: 435 nm: BFP (Blue Fluorescent Protein) 380 nm: 439 nm: Alexa 350: 343 nm: … WebDyLight 550 is a fluorescent compound with an excitation peak at 553 nm and an emission peak at 569 nm. It can be excited using a 561 nm laser paired with a 582/15 nm …

WebCF® Dye Spectrum: CF®350 Dye. CF®350 is a blue fluorescent dye. Learn More. 300 400 500 600 700 800 900 Wavelength (nm) CF350. Technical Summary. ... 555, ATTO 550, Cy®3, DyLight® 549, Rhodamine. Features. Brighter than Cy®3 Highly water-soluble Validated in multicolor STORM super-resolution imaging. WebStorage: Upon receipt store DyLight NHS Esters at -20°C. Products are shipped with an ice pack. Store all dyes in the foil pouch with desiccant to protect from light and moisture. Introduction . The Thermo Scientific DyLight Amine-Reactive Dyes have absorption spectra ranging from 350nm to 770nm (Table 1) and are packaged in a convenient ...

WebFAQs for Lightning-Link (R) Rapid DyLight 550 Kit (323-0005). (Showing 1 - 8 of 8 FAQ). Can I label a peptide/protein/antibody other than IgG? Yes. The Lightning-Link® technology works by targeting free amine groups on your target, meaning it can be used to label most biomolecules.As the protocols provided were optimized for labeling IgGs, we would …

WebTable 2. Properties of the Thermo Scientific DyLight Dyes. DyLight Dye A. max * ε† CF ‡ 350 . 405 . 488 . 550 . 594 . 633 . 650 . 680 . 755 . 800 * Excitation wavelength in nanometers − note that upon protein conjugation the absorption maximum shifts to the right of the spectra †Molar extinction coefficient (M-1. cm-1) at A. max ... fm 101 5 staff organization and operationsWebIHC-P. 1/50 - 1/500. DyLight ® 550 replaces DyLight ® 549. The DyLight ® 550 excitation is 562nm and the emission is 576nm. DyLight ® 550 provides the same orange-to-red fluorescence and photostability as the DyLight ® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight ® 549 or DyLight ® 550. fm100 country in austin mnWebDyLight™ 405 - conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use requires instruments … greensand healthWebStore product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 550 Amax= 562 nm; Emax= 576 nm. Mole Dye/Mole Protein Ratio is lot-dependent. greensand health centre looseWebDyLight™ 650 is a red-emitting fluorophore that excited by the 640 nm laser and collected using a 670/30 nm bandpass filter. DyLight 650 has an excitation peak at 652 nm and an emission peak at 672 nm, and is spectrally similar to Alexa Fluor 647 and Cy5. ... Covering the entire visible light spectrum and several key near-infared and infrared ... greens and hammonds laneWebAlexa Fluor dyes are closely related to DyLight dyes because of structural similarities. Alexa Fluor® 488. Alexa Fluor 488 (Amax 493 nm, Emax 519 nm) has absorption and emission spectra comparable to Fluorescein (FITC), but are much brighter, far more photostable and less sensitive to pH changes between pH 4 and 10. Alexa Fluor 488 conjugates ... fm 101 armyWebThe excitation and emission spectra of the DyLight Fluor series cover much of the visible spectrum and extend into ... (488 nm) laser for DyLight 488, a green (526 nm) laser for … greens and ham hocks recipe slow cooker